Abstract
We report here the identification of the novel subunit of the mitochondrial F1F0-ATPase from Saccharomyces cerevisiae, ATPase subunit e. Yeast ATPase subunit e displays significant similarities in both amino acid sequence, properties (hydropathy and predicted coiled-coil structure) and orientation in the inner membrane, with previously identified mammalian ATPase subunit e proteins. Estimation of its native molecular mass and ability to be co-immunoprecipitated with a subunit of the F1-ATPase, demonstrate that subunit e is a subunit of the F1F0-ATPase. Stable expression of subunit e requires the presence of the mitochondrially encoded subunits of the F0-ATPase. Subunit e had been previously identified as Tim11 and was proposed to be involved in the process of sorting of proteins to the mitochondrial inner membrane.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Blotting, Western
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Carrier Proteins / chemistry*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Chromatography, Gel
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Chromosomes, Fungal / genetics
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Cloning, Molecular
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Down-Regulation
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Fungal Proteins / chemistry
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal
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Membrane Proteins / chemistry*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Mitochondria / enzymology*
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Mitochondrial Proton-Translocating ATPases*
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Molecular Sequence Data
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Precipitin Tests
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Promoter Regions, Genetic
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Protein Conformation
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Proton-Translocating ATPases / chemistry*
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Proton-Translocating ATPases / metabolism
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis
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Sequence Homology, Amino Acid
Substances
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Carrier Proteins
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Fungal Proteins
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Membrane Proteins
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Saccharomyces cerevisiae Proteins
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Mitochondrial Proton-Translocating ATPases
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TIM11 protein, S cerevisiae
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Proton-Translocating ATPases
Associated data
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GENBANK/D13121
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GENBANK/M22350
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GENBANK/M64751
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GENBANK/S52977
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GENBANK/X87671