Yeast mitochondrial F1F0-ATPase: the novel subunit e is identical to Tim11

FEBS Lett. 1997 Jul 14;411(2-3):195-200. doi: 10.1016/s0014-5793(97)00691-1.

Abstract

We report here the identification of the novel subunit of the mitochondrial F1F0-ATPase from Saccharomyces cerevisiae, ATPase subunit e. Yeast ATPase subunit e displays significant similarities in both amino acid sequence, properties (hydropathy and predicted coiled-coil structure) and orientation in the inner membrane, with previously identified mammalian ATPase subunit e proteins. Estimation of its native molecular mass and ability to be co-immunoprecipitated with a subunit of the F1-ATPase, demonstrate that subunit e is a subunit of the F1F0-ATPase. Stable expression of subunit e requires the presence of the mitochondrially encoded subunits of the F0-ATPase. Subunit e had been previously identified as Tim11 and was proposed to be involved in the process of sorting of proteins to the mitochondrial inner membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Western
  • Carrier Proteins / chemistry*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Chromatography, Gel
  • Chromosomes, Fungal / genetics
  • Cloning, Molecular
  • Down-Regulation
  • Fungal Proteins / chemistry
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal
  • Membrane Proteins / chemistry*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Mitochondria / enzymology*
  • Mitochondrial Proton-Translocating ATPases*
  • Molecular Sequence Data
  • Precipitin Tests
  • Promoter Regions, Genetic
  • Protein Conformation
  • Proton-Translocating ATPases / chemistry*
  • Proton-Translocating ATPases / metabolism
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis
  • Sequence Homology, Amino Acid

Substances

  • Carrier Proteins
  • Fungal Proteins
  • Membrane Proteins
  • Saccharomyces cerevisiae Proteins
  • Mitochondrial Proton-Translocating ATPases
  • TIM11 protein, S cerevisiae
  • Proton-Translocating ATPases

Associated data

  • GENBANK/D13121
  • GENBANK/M22350
  • GENBANK/M64751
  • GENBANK/S52977
  • GENBANK/X87671