Abstract
14-3-3 proteins are highly conserved ubiquitous proteins whose explicit functions have remained elusive. Here, we show that the S. cerevisiae 14-3-3 homologs BMH1 and BMH2 are not essential for viability or mating MAPK cascade signaling, but they are essential for pseudohyphal-development MAPK cascade signaling and other processes. Activated alleles of RAS2 and CDC42 induce pseudohyphal development and FG(TyA)-lacZ signaling in Bmh+ strains but not in ste20 (p65PAK) or bmh1 bmh2 mutant strains. Moreover, Bmh1p and Bmh2p associate with Ste20p in vivo. Three alleles of BMH1 encode proteins defective for FG(TyA)-lacZ signaling and association with Ste20p, yet these alleles complement other 14-3-3 functions. Therefore, the 14-3-3 proteins are specifically required for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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14-3-3 Proteins
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Adaptation, Physiological
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Alleles
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Biological Evolution
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Cell Survival / physiology
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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GTP-Binding Proteins / metabolism
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Glycogen / metabolism
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Intracellular Signaling Peptides and Proteins
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Lac Operon / genetics
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MAP Kinase Kinase Kinases
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Molecular Sequence Data
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Mutagenesis / physiology
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Phenotype
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Protein Serine-Threonine Kinases / metabolism
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Protein Structure, Tertiary
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Recombinant Proteins / genetics
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Reproduction / physiology
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / ultrastructure
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Signal Transduction / physiology*
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Species Specificity
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ras Proteins / physiology*
Substances
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14-3-3 Proteins
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BMH1 protein, S cerevisiae
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BMH2 protein, S cerevisiae
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Fungal Proteins
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Intracellular Signaling Peptides and Proteins
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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Glycogen
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Protein Serine-Threonine Kinases
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MAP Kinase Kinase Kinases
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STE20 protein, S cerevisiae
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GTP-Binding Proteins
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ras Proteins