Abstract
Several eukaryotic multiprotein complexes, including the Saccharomyces cerevisiae Snf/Swi complex, remodel chromatin for transcription. In contrast to the Snf/Swi proteins, Sfh1p, a new Snf5p paralog, is essential for viability. The evolutionarily conserved domain of Sfh1p is sufficient for normal function, and Sfh1p interacts functionally and physically with an essential Snf2p paralog in a novel nucleosome-restructuring complex called RSC (for remodels the structure of chromatin). A temperature-sensitive sfh1 allele arrests cells in the G2/M phase of the cell cycle, and the Sfh1 protein is specifically phosphorylated in the G1 phase. Together, these results demonstrate a link between chromatin remodeling and progression through the cell division cycle, providing genetic clues to possible targets for RSC function.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases
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Adenosine Triphosphate / genetics
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Adenosine Triphosphate / metabolism
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Amino Acid Sequence
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Animals
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Caenorhabditis elegans
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Cell Cycle / physiology*
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / physiology*
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Chromatin / metabolism*
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Chromosomal Proteins, Non-Histone*
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Conserved Sequence
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DNA Helicases
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / physiology*
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Drosophila melanogaster
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Fungal Proteins / metabolism
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Humans
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Molecular Sequence Data
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Nuclear Proteins*
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Nucleosomes / metabolism
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Phenotype
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Phosphorylation
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SMARCB1 Protein
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Saccharomyces cerevisiae
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Saccharomyces cerevisiae Proteins*
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription Factors / physiology*
Substances
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Cell Cycle Proteins
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Fungal Proteins
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Nuclear Proteins
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Nucleosomes
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SMARCA2 protein, human
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SMARCB1 Protein
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SMARCB1 protein, human
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SNF5 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Sfh1 protein, S cerevisiae
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Transcription Factors
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Adenosine Triphosphate
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Adenosine Triphosphatases
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SMARCA4 protein, human
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SNF2 protein, S cerevisiae
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STH1 protein, S cerevisiae
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DNA Helicases