Abstract
We isolated a temperature-sensitive mutant of Saccharomyces cerevisiae in which transcription both of ribosomal protein genes and of ribosomal RNA is defective at the non-permissive temperature. Temperature-sensitivity for growth is recessive and segregates 2:2. The wild type gene, termed RIC1 (for ribosome control) was cloned by complementation of the temperature-sensitive phenotype from a genomic DNA library based on the CEN plasmid. RIC1 encodes a protein of 1056 amino acid (aa) residues including a putative nuclear localization sequence. Data base searches revealed that RIC1 is a novel gene and predicted aa sequence share some sequence similarity with viral transcriptional regulatory proteins.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chromosome Mapping
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Cloning, Molecular
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DNA, Fungal
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Fungal Proteins / genetics*
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Genes, Fungal
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Glycoside Hydrolases / metabolism
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Guanine Nucleotide Exchange Factors
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Membrane Proteins
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Molecular Sequence Data
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Mutation
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Nuclear Localization Signals
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Nuclear Proteins / genetics
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RNA, Ribosomal / biosynthesis
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Ribosomal Proteins / genetics
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Ribosomes / metabolism*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Temperature
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Transcription Factors / genetics*
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Transcription, Genetic
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beta-Fructofuranosidase
Substances
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DNA, Fungal
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Fungal Proteins
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Guanine Nucleotide Exchange Factors
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Membrane Proteins
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Nuclear Localization Signals
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Nuclear Proteins
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RIC1 protein, S cerevisiae
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RNA, Ribosomal
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Ribosomal Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Glycoside Hydrolases
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beta-Fructofuranosidase