Abstract
The Saccharomyces cerevisiae SPE3 gene, coding for spermidine synthase, was cloned, sequenced, and localized on the right arm of chromosome XVI. The deduced amino acid sequence has a high similarity to mammalian spermidine synthases, and has putative S-adenosylmethionine binding motifs. To investigate the effect of total loss of the SPE3 gene, we constructed a null mutant of this gene, spe3delta, which has no spermidine synthase activity and has an absolute requirement for spermidine or spermine for the growth. This requirement is satisfied by a very low concentration of spermidine (10(-8) M) or a higher concentration of spermine (10(-6) M).
MeSH terms
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Amino Acid Sequence
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Chromosome Mapping
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Cloning, Molecular
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Conserved Sequence
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Deoxyadenosines / metabolism
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Genes, Fungal*
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Molecular Sequence Data
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Mutation
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S-Adenosylmethionine / analogs & derivatives
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S-Adenosylmethionine / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism*
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Sequence Analysis
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Sequence Homology, Amino Acid
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Spermidine / biosynthesis*
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Spermidine / metabolism
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Spermidine Synthase / genetics*
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Thionucleosides / metabolism
Substances
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Deoxyadenosines
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Thionucleosides
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S-adenosyl-3-methylthiopropylamine
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5'-methylthioadenosine
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S-Adenosylmethionine
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Spermidine Synthase
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Spermidine