Abstract
The DED1 gene, which encodes a putative RNA helicase, has been implicated in nuclear pre-messenger RNA splicing in the yeast Saccharomyces cerevisiae. It is shown here by genetic and biochemical analysis that translation, rather than splicing, is severely impaired in two newly isolated ded1 conditional mutants. Preliminary evidence suggests that the protein Ded1p may be required for the initiation step of translation, as is the distinct DEAD-box protein, eukaryotic initiation factor 4A (eIF4A). The DED1 gene could be functionally replaced by a mouse homolog, PL10, which suggests that the function of Ded1p in translation is evolutionarily conserved.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cytoplasm / metabolism
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DEAD-box RNA Helicases
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Eukaryotic Initiation Factor-4A
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Genes, Fungal
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Mice
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Mutation
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Peptide Initiation Factors / genetics
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Peptide Initiation Factors / metabolism
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Phenotype
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Protein Biosynthesis*
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RNA Helicases
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RNA Nucleotidyltransferases / genetics
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RNA Nucleotidyltransferases / metabolism*
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RNA Splicing
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RNA, Fungal / genetics*
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RNA, Messenger / genetics*
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
Substances
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Peptide Initiation Factors
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RNA, Fungal
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RNA, Messenger
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Eukaryotic Initiation Factor-4A
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RNA Nucleotidyltransferases
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D1Pas1 protein, mouse
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DBP1 protein, S cerevisiae
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DEAD-box RNA Helicases
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RNA Helicases