Abstract
A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWII/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / metabolism
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Adenosine Triphosphatases / chemistry*
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Adenosine Triphosphate / metabolism
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Amino Acid Sequence
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Cell Cycle Proteins*
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Chromatin / ultrastructure*
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Chromosomal Proteins, Non-Histone / physiology*
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DNA Helicases*
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DNA-Binding Proteins / physiology*
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Fungal Proteins / physiology*
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Gene Expression Regulation, Fungal
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Genes, Fungal
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Histone Acetyltransferases
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Macromolecular Substances
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Mass Spectrometry
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Molecular Sequence Data
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Nuclear Proteins / physiology*
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Nucleosomes / ultrastructure*
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Protein Structure, Secondary
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RNA Polymerase II / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Trans-Activators*
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Transcription Factors / chemistry*
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Transcription Factors / physiology*
Substances
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Cell Cycle Proteins
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Fungal Proteins
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Macromolecular Substances
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Nuclear Proteins
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Nucleosomes
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RSC complex, S cerevisiae
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SNF12 protein, S cerevisiae
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SWI3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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Transcription Factors
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Adenosine Triphosphate
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Acetyltransferases
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Histone Acetyltransferases
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RNA Polymerase II
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Adenosine Triphosphatases
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SNF2 protein, S cerevisiae
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STH1 protein, S cerevisiae
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DNA Helicases