As in Saccharomyces cerevisiae, aspartate transcarbamoylase is assembled on a multifunctional protein including a dihydroorotase-like cryptic domain in Schizosaccharomyces pombe

Curr Genet. 1995 Jul;28(2):138-49. doi: 10.1007/BF00315780.

Abstract

The organisation of the URA1 gene of Schizosaccharomyces pombe was determined from the entire cDNA cloned by the transformation of an ATCase-deficient strain of Saccharomyces cerevisiae. The URA1 gene encodes the bifunctional protein GLNase/CPSase-ATCase which catalyses the first two steps of the pyrimidine biosynthesis pathway. The complete nucleotide sequence of the URA1 cDNA was elucidated and the deduced amino-acid sequence was used to define four domains in the protein; three functional domains, corresponding to GLNase (glutamine amidotransferase), CPSase (carbamoylphosphate synthetase) and ATCase (aspartate transcarbamoylase) activities, and one cryptic DHOase (dihydroorotase) domain. Genetic investigations confirmed that both GLNase/CPSase and ATCase activities are carried out by the same polypeptide. They are also both feedback-inhibited by UTP (uridine triphosphate). Its organization and regulation indicate that the S. pombe URA1 gene product appears very similar to the S. cerevisiae URA2 gene product.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anthranilate Synthase*
  • Aspartate Carbamoyltransferase / antagonists & inhibitors
  • Aspartate Carbamoyltransferase / genetics*
  • Aspartate Carbamoyltransferase / metabolism
  • Base Sequence
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / antagonists & inhibitors
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / genetics*
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / metabolism
  • Cloning, Molecular
  • DNA, Fungal
  • Dihydroorotase / metabolism*
  • Molecular Sequence Data
  • Multienzyme Complexes / antagonists & inhibitors
  • Multienzyme Complexes / genetics*
  • Multienzyme Complexes / metabolism
  • Nitrogenous Group Transferases*
  • Saccharomyces cerevisiae / enzymology*
  • Schizosaccharomyces / enzymology
  • Schizosaccharomyces / genetics*
  • Sequence Homology, Amino Acid
  • Transferases / metabolism
  • Uridine Triphosphate / metabolism

Substances

  • DNA, Fungal
  • Multienzyme Complexes
  • carbamoyl phosphate synthase-aspartatecarbamoyl transferase complex
  • Transferases
  • Aspartate Carbamoyltransferase
  • Nitrogenous Group Transferases
  • Dihydroorotase
  • Anthranilate Synthase
  • anthranilate synthase, glutamine amidotransferase subunit
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
  • Uridine Triphosphate

Associated data

  • GENBANK/M27174
  • GENBANK/X81841
  • SWISSPROT/P08955
  • SWISSPROT/P20054