Abstract
The organisation of the URA1 gene of Schizosaccharomyces pombe was determined from the entire cDNA cloned by the transformation of an ATCase-deficient strain of Saccharomyces cerevisiae. The URA1 gene encodes the bifunctional protein GLNase/CPSase-ATCase which catalyses the first two steps of the pyrimidine biosynthesis pathway. The complete nucleotide sequence of the URA1 cDNA was elucidated and the deduced amino-acid sequence was used to define four domains in the protein; three functional domains, corresponding to GLNase (glutamine amidotransferase), CPSase (carbamoylphosphate synthetase) and ATCase (aspartate transcarbamoylase) activities, and one cryptic DHOase (dihydroorotase) domain. Genetic investigations confirmed that both GLNase/CPSase and ATCase activities are carried out by the same polypeptide. They are also both feedback-inhibited by UTP (uridine triphosphate). Its organization and regulation indicate that the S. pombe URA1 gene product appears very similar to the S. cerevisiae URA2 gene product.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anthranilate Synthase*
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Aspartate Carbamoyltransferase / antagonists & inhibitors
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Aspartate Carbamoyltransferase / genetics*
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Aspartate Carbamoyltransferase / metabolism
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Base Sequence
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Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / antagonists & inhibitors
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Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / genetics*
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Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / metabolism
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Cloning, Molecular
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DNA, Fungal
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Dihydroorotase / metabolism*
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Molecular Sequence Data
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Multienzyme Complexes / antagonists & inhibitors
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Multienzyme Complexes / genetics*
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Multienzyme Complexes / metabolism
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Nitrogenous Group Transferases*
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Saccharomyces cerevisiae / enzymology*
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Schizosaccharomyces / enzymology
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Schizosaccharomyces / genetics*
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Sequence Homology, Amino Acid
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Transferases / metabolism
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Uridine Triphosphate / metabolism
Substances
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DNA, Fungal
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Multienzyme Complexes
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carbamoyl phosphate synthase-aspartatecarbamoyl transferase complex
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Transferases
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Aspartate Carbamoyltransferase
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Nitrogenous Group Transferases
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Dihydroorotase
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Anthranilate Synthase
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anthranilate synthase, glutamine amidotransferase subunit
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Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
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Uridine Triphosphate
Associated data
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GENBANK/M27174
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GENBANK/X81841
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SWISSPROT/P08955
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SWISSPROT/P20054