Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: biochemical and molecular characterization of both hexokinases

FEBS Lett. 1996 Jan 8;378(2):185-9. doi: 10.1016/0014-5793(95)01451-9.

Abstract

Two hexokinases were characterized in Schizosaccharomyces pombe: hexokinase 1, with a low phosphorylation coefficient on glucose (Km 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1+ and hxk2+ encoding these enzymes were cloned and sequenced. Disruption of hxk1+ had no effect on growth but disruption of hxk2+ doubled the generation time in glucose. Spores carrying the double disruption hxk1+ hxk2+ did not grow on glucose or fructose after one week. Expression of hxk1+ increased strongly during growth in fructose or glycerol. Expression of hxk2+ was highest during growth in glycerol. A NADP-dependent glucose dehydrogenase was detected, but not a glucokinase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • Fructose / metabolism
  • Gene Expression
  • Genes, Fungal
  • Glucose / metabolism
  • Glucose 1-Dehydrogenase
  • Glucose Dehydrogenases / metabolism
  • Hexokinase / chemistry
  • Hexokinase / genetics
  • Hexokinase / metabolism*
  • Molecular Sequence Data
  • NADP / pharmacology
  • Phosphorylation
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Schizosaccharomyces / enzymology*
  • Sequence Analysis, DNA
  • Sequence Homology

Substances

  • RNA, Messenger
  • Fructose
  • NADP
  • Glucose Dehydrogenases
  • Glucose 1-Dehydrogenase
  • Hexokinase
  • Glucose

Associated data

  • GENBANK/X92894
  • GENBANK/X92895