Abstract
The ENA2 gene encoding a P-type ATPase involved in Na+ and Li+ effluxes in Saccharomyces cerevisiae has been isolated. The putative protein encoded by ENA2 differs only in thirteen amino acids from the protein encoded by ENA1/PMR2. However, ENA2 has a very low level of expression and for this reason did not confer significant Li+ tolerance on a Li+ sensitive strain. ENA1 and ENA2 are the first two units of a tandem array of four highly homologous genes with probably homologous functions.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / genetics*
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Base Sequence
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DNA, Recombinant
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Drug Resistance, Microbial
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Gene Expression Regulation, Enzymologic*
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Genes, Fungal / genetics*
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Isoenzymes / genetics*
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Lac Operon / genetics
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Lithium / metabolism
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Lithium / pharmacology
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Molecular Sequence Data
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Plasmids / genetics
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Saccharomyces cerevisiae / genetics*
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Sodium / metabolism*
Substances
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DNA, Recombinant
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Isoenzymes
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Lithium
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Sodium
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Adenosine Triphosphatases