Abstract
A Saccharomyces cerevisiae cDNA homologue of the elongation factor EF-1 beta was found among the clones obtained by immunoscreening of a yeast cDNA expression library with an antibody against calmodulin affinity-purified proteins. The cDNA encoded a protein of 206 amino acids which was highly homologous (about 70% homology) with Artemia salina and human EF-1 beta. A protein with an apparent molecular mass of 33,000, significantly larger than that expected from the gene, was identified by Western blotting. Gene disruption experiments indicated that EF-1 beta is essential for growth.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Southern
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Blotting, Western
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Cloning, Molecular
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DNA, Fungal
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Genes, Fungal
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Humans
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Molecular Sequence Data
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Peptide Elongation Factor 1
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Peptide Elongation Factors / genetics*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Sequence Homology, Amino Acid
Substances
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DNA, Fungal
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Peptide Elongation Factor 1
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Peptide Elongation Factors
Associated data
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GENBANK/D14080
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GENBANK/L22015
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GENBANK/X68456
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GENBANK/X68457
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GENBANK/X68458
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GENBANK/X68459
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GENBANK/X68460
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GENBANK/X68461
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GENBANK/X68462
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GENBANK/X68463