Cloning and characterization of the elongation factor EF-1 beta homologue of Saccharomyces cerevisiae. EF-1 beta is essential for growth

FEBS Lett. 1993 Jan 25;316(2):165-9. doi: 10.1016/0014-5793(93)81208-h.

Abstract

A Saccharomyces cerevisiae cDNA homologue of the elongation factor EF-1 beta was found among the clones obtained by immunoscreening of a yeast cDNA expression library with an antibody against calmodulin affinity-purified proteins. The cDNA encoded a protein of 206 amino acids which was highly homologous (about 70% homology) with Artemia salina and human EF-1 beta. A protein with an apparent molecular mass of 33,000, significantly larger than that expected from the gene, was identified by Western blotting. Gene disruption experiments indicated that EF-1 beta is essential for growth.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Blotting, Western
  • Cloning, Molecular
  • DNA, Fungal
  • Genes, Fungal
  • Humans
  • Molecular Sequence Data
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors / genetics*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Sequence Homology, Amino Acid

Substances

  • DNA, Fungal
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors

Associated data

  • GENBANK/D14080
  • GENBANK/L22015
  • GENBANK/X68456
  • GENBANK/X68457
  • GENBANK/X68458
  • GENBANK/X68459
  • GENBANK/X68460
  • GENBANK/X68461
  • GENBANK/X68462
  • GENBANK/X68463