Abstract
A gene encoding a functional acetolactate synthase (ALS) subunit has been isolated from the fission yeast Schizosaccharomyces pombe, and has been structurally and genetically characterized. The approximate 5-kbp cloned DNA segment was found to contain a 2007-bp open reading frame capable of encoding a 669 aminoacid polypeptide which exhibited 57.1% similarity to the corresponding ALS subunit from Saccharomyces cerevisiae. The putative ilv1 isolated from S. pombe was shown to encode a functional subunit of acetolactate synthase by complementation of an S. cerevisiae strain deleted for the ILV2 locus.
MeSH terms
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Acetolactate Synthase / genetics*
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Amino Acid Sequence
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Base Sequence
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DNA Primers
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DNA, Fungal / isolation & purification
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DNA, Fungal / metabolism
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Genes, Fungal
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Genetic Complementation Test
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Macromolecular Substances
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Molecular Sequence Data
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Open Reading Frames
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Point Mutation*
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Polymerase Chain Reaction
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Schizosaccharomyces / enzymology*
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Schizosaccharomyces / genetics*
Substances
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DNA Primers
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DNA, Fungal
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Macromolecular Substances
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Acetolactate Synthase
Associated data
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GENBANK/L11293
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GENBANK/X70022
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GENBANK/X70023
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GENBANK/X70024
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GENBANK/X70025
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GENBANK/X70026
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GENBANK/X70027
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GENBANK/X70028
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GENBANK/X71679
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GENBANK/X71680