Isolation and structure of an acetolactate synthase gene from Schizosaccharomyces pombe and complementation of the ilv2 mutation in Saccharomyces cerevisiae

Curr Genet. 1993 Dec;24(6):544-7. doi: 10.1007/BF00351720.

Abstract

A gene encoding a functional acetolactate synthase (ALS) subunit has been isolated from the fission yeast Schizosaccharomyces pombe, and has been structurally and genetically characterized. The approximate 5-kbp cloned DNA segment was found to contain a 2007-bp open reading frame capable of encoding a 669 aminoacid polypeptide which exhibited 57.1% similarity to the corresponding ALS subunit from Saccharomyces cerevisiae. The putative ilv1 isolated from S. pombe was shown to encode a functional subunit of acetolactate synthase by complementation of an S. cerevisiae strain deleted for the ILV2 locus.

Publication types

  • Comparative Study

MeSH terms

  • Acetolactate Synthase / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • DNA Primers
  • DNA, Fungal / isolation & purification
  • DNA, Fungal / metabolism
  • Genes, Fungal
  • Genetic Complementation Test
  • Macromolecular Substances
  • Molecular Sequence Data
  • Open Reading Frames
  • Point Mutation*
  • Polymerase Chain Reaction
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Schizosaccharomyces / enzymology*
  • Schizosaccharomyces / genetics*

Substances

  • DNA Primers
  • DNA, Fungal
  • Macromolecular Substances
  • Acetolactate Synthase

Associated data

  • GENBANK/L11293
  • GENBANK/X70022
  • GENBANK/X70023
  • GENBANK/X70024
  • GENBANK/X70025
  • GENBANK/X70026
  • GENBANK/X70027
  • GENBANK/X70028
  • GENBANK/X71679
  • GENBANK/X71680