Abstract
We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA endonuclease (SPDE) catalyzes a single ATP-independent incision immediately 5' to the UV photoproduct and generates termini containing 3' hydroxyl and 5' phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recognition/cleavage step of a DNA excision repair pathway.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / pharmacology*
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Base Sequence
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DNA / chemistry
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DNA / metabolism
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DNA Damage
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DNA Repair*
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Endodeoxyribonucleases / metabolism*
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Molecular Sequence Data
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Photochemistry
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Pyrimidine Dimers / metabolism*
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Schizosaccharomyces / enzymology*
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Schizosaccharomyces pombe Proteins*
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Substrate Specificity
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Ultraviolet Rays
Substances
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Pyrimidine Dimers
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Schizosaccharomyces pombe Proteins
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Adenosine Triphosphate
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DNA
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Endodeoxyribonucleases
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uve1 protein, S pombe