The UGA4 UASNTR site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding in Saccharomyces cerevisiae

J Bacteriol. 1994 Aug;176(15):4718-25. doi: 10.1128/jb.176.15.4718-4725.1994.

Abstract

Expression of the nitrogen catabolic genes in Saccharomyces cerevisiae, including those of the gamma-aminobutyric acid (UGA) and allantoin (DAL) pathways, is regulated positively by the GLN3 protein and negatively by the DAL80 protein. The deduced sequences of the DAL80 and GLN3 proteins contain a zinc finger motif homologous to those shown to bind GATA sequences. In addition, DAL80 protein has been directly shown to bind to a pair of GATA-containing sequences (URSGATA) in vitro, and a pair of GATA-containing sequences (UASNTR) is required for GLN3-dependent transcriptional activation in a heterologous expression vector. We demonstrate here that the GATA-containing sites upstream of UGA4 required for optimal GLN3-dependent transcriptional activation also mediate DAL80 protein binding in vitro and DAL80-responsive regulation in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 4-Aminobutyrate Transaminase / genetics
  • Allantoin / metabolism
  • Base Sequence
  • Carrier Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism*
  • GABA Plasma Membrane Transport Proteins
  • GATA Transcription Factors
  • Gene Expression Regulation, Fungal
  • Membrane Transport Proteins / genetics*
  • Membrane Transport Proteins / metabolism*
  • Molecular Sequence Data
  • Organic Anion Transporters*
  • Protein Binding
  • Regulatory Sequences, Nucleic Acid / genetics*
  • Repressor Proteins / metabolism*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors*
  • Transcription, Genetic
  • gamma-Aminobutyric Acid / metabolism

Substances

  • Carrier Proteins
  • DAL80 protein, S cerevisiae
  • DNA-Binding Proteins
  • Fungal Proteins
  • GABA Plasma Membrane Transport Proteins
  • GATA Transcription Factors
  • GLN3 protein, S cerevisiae
  • Membrane Transport Proteins
  • Organic Anion Transporters
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • UGA4 protein, S cerevisiae
  • Allantoin
  • gamma-Aminobutyric Acid
  • 4-Aminobutyrate Transaminase

Associated data

  • GENBANK/L28080
  • GENBANK/L28081