Abstract
Expression of the nitrogen catabolic genes in Saccharomyces cerevisiae, including those of the gamma-aminobutyric acid (UGA) and allantoin (DAL) pathways, is regulated positively by the GLN3 protein and negatively by the DAL80 protein. The deduced sequences of the DAL80 and GLN3 proteins contain a zinc finger motif homologous to those shown to bind GATA sequences. In addition, DAL80 protein has been directly shown to bind to a pair of GATA-containing sequences (URSGATA) in vitro, and a pair of GATA-containing sequences (UASNTR) is required for GLN3-dependent transcriptional activation in a heterologous expression vector. We demonstrate here that the GATA-containing sites upstream of UGA4 required for optimal GLN3-dependent transcriptional activation also mediate DAL80 protein binding in vitro and DAL80-responsive regulation in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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4-Aminobutyrate Transaminase / genetics
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Allantoin / metabolism
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Base Sequence
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Carrier Proteins / genetics*
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism*
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GABA Plasma Membrane Transport Proteins
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GATA Transcription Factors
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Gene Expression Regulation, Fungal
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Membrane Transport Proteins / genetics*
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Membrane Transport Proteins / metabolism*
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Molecular Sequence Data
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Organic Anion Transporters*
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Protein Binding
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Regulatory Sequences, Nucleic Acid / genetics*
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Repressor Proteins / metabolism*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Transcription Factors*
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Transcription, Genetic
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gamma-Aminobutyric Acid / metabolism
Substances
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Carrier Proteins
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DAL80 protein, S cerevisiae
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DNA-Binding Proteins
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Fungal Proteins
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GABA Plasma Membrane Transport Proteins
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GATA Transcription Factors
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GLN3 protein, S cerevisiae
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Membrane Transport Proteins
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Organic Anion Transporters
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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UGA4 protein, S cerevisiae
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Allantoin
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gamma-Aminobutyric Acid
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4-Aminobutyrate Transaminase
Associated data
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GENBANK/L28080
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GENBANK/L28081