Purification of FKBP-70, a novel immunophilin from Saccharomyces cerevisiae, and cloning of its structural gene, FPR3

FEBS Lett. 1994 Sep 19;352(1):98-103. doi: 10.1016/0014-5793(94)00927-9.

Abstract

A novel protein, belonging to the yeast family of FKBPs (FK-binding proteins), FKBP-70, was isolated from Saccharomyces cerevisiae by its interaction with the immunosuppressive agent FK-520. Its structural gene, FPR3, was cloned and the protein expressed and purified from Escherichia coli. This third member of the FKBP family in yeast is homologous to the other FKBPs at its carboxy terminus, showing conserved ligand binding and proline isomerase regions. It is, however, a longer acidic protein with several potential nuclear targeting sequences and a region of homology to nucleolins. Yeast strains deleted for FPR3, as well as a triple deletion mutant of this family of genes, FPR1, FPR2 and FPR3, are viable under normal conditions of growth, indicating that the FPR genes are not essential for life.

MeSH terms

  • Amino Acid Isomerases / metabolism
  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / metabolism
  • Cloning, Molecular
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / metabolism
  • Gene Expression
  • Genes, Fungal / genetics*
  • Heat-Shock Proteins / chemistry
  • Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / isolation & purification
  • Heat-Shock Proteins / metabolism
  • Molecular Sequence Data
  • Molecular Weight
  • Peptidylprolyl Isomerase
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / genetics*
  • Sequence Alignment
  • Sequence Analysis
  • Sequence Analysis, DNA
  • Sequence Deletion / physiology
  • Sequence Homology, Amino Acid
  • Tacrolimus Binding Proteins

Substances

  • Carrier Proteins
  • Fungal Proteins
  • Heat-Shock Proteins
  • Recombinant Fusion Proteins
  • Amino Acid Isomerases
  • Tacrolimus Binding Proteins
  • Peptidylprolyl Isomerase

Associated data

  • GENBANK/S73876