A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction

Science. 1995 Feb 24;267(5201):1166-9. doi: 10.1126/science.7855597.

Abstract

Exonuclease I (Exo I) from Schizosaccharomyces pombe, a 5'-->3' double-stranded DNA exonuclease, is induced during meiotic prophase I. The exo1 gene is a member of a family of related DNA repair genes, including RAD2/rad13/xpgc and YKL510/rad2, conserved from yeast to humans. An exo1 mutant displays a mutator phenotype and alters activity of the ade6-M387 marker effect. These results suggest that Exo I acts in a pathway that corrects mismatched base pairs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Composition
  • Base Sequence
  • Crosses, Genetic
  • DNA Repair*
  • DNA Replication
  • DNA, Fungal / metabolism*
  • Exodeoxyribonucleases / chemistry
  • Exodeoxyribonucleases / genetics
  • Exodeoxyribonucleases / metabolism*
  • Meiosis
  • Molecular Sequence Data
  • Mutation*
  • Recombination, Genetic
  • Schizosaccharomyces / enzymology
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / physiology

Substances

  • DNA, Fungal
  • Exodeoxyribonucleases
  • exodeoxyribonuclease I

Associated data

  • GENBANK/L35174