Abstract
The protein kinase-encoding genes RCK1 and RCK2 from Saccharomyces cerevisiae have been identified as suppressors of Schizosaccharomyces pombe cell cycle checkpoint mutations. Upon expression of these genes, radiation resistance is partially restored in S. pombe mutants with checkpoint deficiencies, but not in mutants with DNA repair defects. Some checkpoint mutants are sensitive to the DNA synthesis inhibitor hydroxyurea, and this sensitivity is also suppressed by RCK1 and RCK2. The degree of suppression can be modulated by varying expression levels. Expression of RCK1 or RCK2 in S. pombe causes cell elongation and decelerated growth. Cells expressing these genes have a single nucleus and a 2n DNA content. We conclude that these genes act in S. pombe to prolong the G2 phase of the cell cycle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cloning, Molecular
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DNA Repair / genetics
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DNA Repair Enzymes
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DNA, Fungal / antagonists & inhibitors
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DNA, Fungal / biosynthesis
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DNA-Binding Proteins*
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Endonucleases*
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Fungal Proteins / genetics
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G2 Phase / genetics*
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Gamma Rays
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Genes, Fungal / genetics*
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Hydroxyurea / pharmacology
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Molecular Sequence Data
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Protein Serine-Threonine Kinases / genetics*
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces / cytology
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Schizosaccharomyces / drug effects
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Schizosaccharomyces / enzymology
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Schizosaccharomyces / genetics*
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Schizosaccharomyces pombe Proteins
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Sequence Analysis, DNA
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Suppression, Genetic / genetics*
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Ultraviolet Rays
Substances
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DNA, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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RCK2 protein, S cerevisiae
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Protein Serine-Threonine Kinases
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RCK1 protein, S cerevisiae
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Endonucleases
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RAD1 protein, S cerevisiae
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rad1 protein, S pombe
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DNA Repair Enzymes
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Hydroxyurea