Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor

Science. 1995 Jul 28;269(5223):554-8. doi: 10.1126/science.7624781.

Abstract

The role of mitogen-activated protein (MAP) kinase cascades in integrating distinct upstream signals was studied in yeast. Mutants that were not able to activate PBS2 MAP kinase kinase (MAPKK; Pbs2p) at high osmolarity were characterized. Pbs2p was activated by two independent signals that emanated from distinct cell-surface osmosensors. Pbs2p was activated by MAP kinase kinase kinases (MAPKKKs) Ssk2p and Ssk22p that are under the control of the SLN1-SSK1 two-component osmosensor. Alternatively, Pbs2p was activated by a mechanism that involves the binding of its amino terminal proline-rich motif to the Src homology 3 (SH3) domain of a putative transmembrane osmosensor Sho1p.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Cloning, Molecular
  • Enzyme Activation
  • Fungal Proteins / metabolism
  • Genes, Fungal
  • Intracellular Signaling Peptides and Proteins
  • MAP Kinase Kinase Kinases
  • Mitogen-Activated Protein Kinase Kinases
  • Mitogen-Activated Protein Kinases*
  • Molecular Sequence Data
  • Mutation
  • Osmolar Concentration
  • Phosphorylation
  • Protein Kinases / metabolism*
  • Protein Serine-Threonine Kinases / metabolism*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Signal Transduction

Substances

  • Fungal Proteins
  • Intracellular Signaling Peptides and Proteins
  • SSK1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Protein Kinases
  • Protein Serine-Threonine Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • HOG1 protein, S cerevisiae
  • Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase Kinases
  • Mitogen-Activated Protein Kinase Kinases
  • SLN1 protein, S cerevisiae

Associated data

  • GENBANK/L41926
  • GENBANK/L41927