Isolation and characterization of Saccharomyces cerevisiae glycolytic pathway mutants

J Bacteriol. 1977 May;130(2):746-9. doi: 10.1128/jb.130.2.746-749.1977.

Abstract

Yeast strains carrying recessive mutations representing four different loci that cause defects in pyruvate kinase, pyruvate decarboxylase, 3-phosphoglycerate kinase, and 3-phosphoglycerate mutase were isolated and partially characterized. Cells carrying these mutations were unable to use glucose as a carbon source as measured in turbidimetric growth experiments. Tetrad analysis indicated that these mutations were not linked to each other; one of the mutations, that affecting phosphoglycerate kinase, was located on chromosome III.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping
  • Culture Media
  • Galactose / metabolism
  • Gluconeogenesis
  • Glucose / metabolism
  • Glycolysis*
  • Mutation*
  • Oxidative Phosphorylation
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*

Substances

  • Culture Media
  • Glucose
  • Galactose