ATP4, the structural gene for yeast F0F1 ATPase subunit 4

Eur J Biochem. 1988 Jan 4;170(3):637-42. doi: 10.1111/j.1432-1033.1988.tb13745.x.

Abstract

A plasmid containing the gene coding for the Saccharomyces cerevisiae F0F1 ATPase subunit 4 was isolated from a yeast genomic DNA library using the oligonucleotide probe procedure. The gene and the surrounding regions were cloned into M13 tg 130 and M13 tg 131 phage vectors. A 732-base-pair open reading frame encoding a 244-amino-acid polypeptide is described. The nucleotide sequence predicts that subunit 4 is probably derived from a precursor protein with a hydrophilic and basic 35-amino-acid leader sequence. Mature subunit 4 contains 209 amino acid residues and the predicted molecular mass is 23250 Da. This subunit presents amphiphilic behaviour with two distinct domains. A high alpha-helix content of 77% was predicted from the sequence. Subunit 4 shows homology with the b subunit of Escherichia coli ATP synthase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Escherichia coli / genetics
  • Genes*
  • Genes, Fungal*
  • Macromolecular Substances
  • Molecular Sequence Data
  • Proton-Translocating ATPases / genetics*
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Sequence Homology, Nucleic Acid

Substances

  • Macromolecular Substances
  • DNA Restriction Enzymes
  • Proton-Translocating ATPases

Associated data

  • GENBANK/X06732