Isolation of the yeast INO4 gene, a positive regulator of phospholipid biosynthesis

Curr Genet. 1988;13(1):7-14. doi: 10.1007/BF00365749.

Abstract

Yeast ino4 mutants are auxotrophic for the phospholipid precursor inositol and have pleiotropic defects in phospholipid synthesis. The mutants are unable to derepress the cytoplasmic enzyme, inositol-1-phosphate synthase and they exhibit reduced synthesis of methylated phospholipids, particularly phosphatidyl-choline. The INO4 gene is believed to encode a positive regulator involved in coordinate control of phospholipid synthesis. In the present study, we report the isolation of two clones containing the INO4 gene. The clones share a region of homology and were mapped to the INO4 locus. Southern blot analysis revealed that the cloned DNA contained both unique and repetitive DNA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crosses, Genetic
  • DNA Restriction Enzymes
  • Genes, Fungal*
  • Genes, Regulator*
  • Mutation
  • Nucleotide Mapping
  • Phospholipids / biosynthesis*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Phospholipids
  • DNA Restriction Enzymes