Cloning of the glutamine:fructose-6-phosphate amidotransferase gene from yeast. Pheromonal regulation of its transcription

J Biol Chem. 1989 May 25;264(15):8753-8.

Abstract

The activity of the amino sugar-synthesizing enzyme L-glutamine:D-fructose-6-phosphate aminotransferase (EC 2.6.1.16) in haploid a cells of Saccharomyces cerevisiae increases 1.7-fold after alpha factor addition. The gene (the gene should be called GFA1 for glutamine:fructose-6-phosphate amidotransferase) for the enzyme has been cloned by complementing the gcn1 mutation (Whelan, W. L., and Ballou, C. E. (1975) J. Bacteriol. 125, 1545-1557). Its expression is increased 2-3 times within 15 min when the mating pheromone is added. The gene codes for a protein of 716 amino acids in length. It is highly homologous (64%) to the corresponding gene of Escherichia coli, except for a sequence coding for 83 amino acids (numbers 204-286), which is lacking in E. coli. The amino-terminal region of the coding sequence also shows a high degree of homology to the corresponding sequence of the E. coli and S. cerevisiae L-glutamine:phosphoribosylpyrophosphate amidotransferase. In the promotor region of the S. cerevisiae L-glutamine:D-fructose-6-phosphate amidotransferase gene the heptanucleotide "TGAAACA," shown to be required for pheromone control of transcription (Kronstad, J. W., Holly, J. A., and MacKay, V. L. (1987) Cell 50, 369-377), is present six times.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Genes*
  • Genes, Fungal*
  • Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / genetics*
  • Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / metabolism
  • Mating Factor
  • Molecular Sequence Data
  • Peptides / physiology*
  • Pheromones / physiology
  • RNA, Messenger / genetics
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Transaminases / genetics*
  • Transcription, Genetic*

Substances

  • Peptides
  • Pheromones
  • RNA, Messenger
  • Mating Factor
  • Transaminases
  • Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)

Associated data

  • GENBANK/J04719