A yeast mutant lacking mitochondrial porin is respiratory-deficient, but can recover respiration with simultaneous accumulation of an 86-kd extramitochondrial protein

EMBO J. 1987 Mar;6(3):723-8. doi: 10.1002/j.1460-2075.1987.tb04813.x.

Abstract

A yeast mutant lacking the only known pore-forming protein of the mitochondrial outer membrane was constructed by gene disruption. The mutant retained all other major proteins of the mitochondrial outer membrane, but was severely deficient in mitochondrial cytochromes and initially did not grow on the non-fermentable carbon source, glycerol. However, it could slowly adapt to glycerol; adaptation was accompanied by the partial restoration of cytochrome levels and massive accumulation of an 86-kd polypeptide in extramitochondrial cell fractions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / genetics*
  • DNA, Recombinant / metabolism
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Genes*
  • Genes, Fungal*
  • Ion Channels / metabolism
  • Mitochondria / metabolism*
  • Molecular Weight
  • Mutation*
  • Oxygen Consumption*
  • Porins
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism

Substances

  • Bacterial Outer Membrane Proteins
  • DNA, Recombinant
  • Fungal Proteins
  • Ion Channels
  • Porins