Proline-independent binding of PUT3 transcriptional activator protein detected by footprinting in vivo

Mol Cell Biol. 1991 Jan;11(1):564-7. doi: 10.1128/mcb.11.1.564-567.1991.

Abstract

The PUT3 gene product is a transcriptional activator required for expression of the enzymes of the proline utilization pathway. Using two methods of footprinting in vivo, we have determined that PUT3 protein is poised at the promoters of the genes encoding these enzymes and that proline-mediated induction modulates the activity of constitutively bound PUT3.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / metabolism*
  • Proline / metabolism*
  • Promoter Regions, Genetic*
  • Regulatory Sequences, Nucleic Acid*
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Transcription Factors / metabolism*

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • Transcription Factors
  • Proline