Abstract
I have cloned a yeast gene, RGM1, which encodes a proline-rich zinc, finger protein. rgm1 mutants do not show any obvious phenotype but overexpression of RGM1 gene greatly impairs cell growth. The proline-rich region of RGM1 attached to a heterologous DNA binding domain is able to repress the expression of the target gene. RGM1 shares similar zinc finger motifs with the mammalian Egr (early growth response) proteins as well as proline-rich sequences with a high serine and threonine content, suggesting that RGM1 and Egr proteins could have functional similarities.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carbohydrate Metabolism
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Cloning, Molecular
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Gene Expression
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Molecular Sequence Data
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Mutagenesis
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Plasmids
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / physiology
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Repressor Proteins / chemistry
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Repressor Proteins / genetics*
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Restriction Mapping
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Sequence Homology, Nucleic Acid
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Transfection
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Zinc Fingers / genetics*
Substances
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Recombinant Fusion Proteins
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Repressor Proteins
Associated data
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GENBANK/S54205
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GENBANK/S54209
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GENBANK/S54211
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GENBANK/S54215
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GENBANK/S54217
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GENBANK/S54227
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GENBANK/S54228
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GENBANK/S54230
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GENBANK/S57650
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GENBANK/X59861