Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases

Genes Dev. 1991 Jan;5(1):60-73. doi: 10.1101/gad.5.1.60.

Abstract

Staurosporine, a potent inhibitor of protein kinase C, arrests fission yeast cell elongation specifically at a stage immediately after cell division. We isolated two genes, which, when carried on multicopy plasmids, confer drug resistance in fission yeast. One, spk1+, encodes a protein kinase highly similar (54% identity) to those encoded by the mammalian ERK1/MAP2 kinase and the budding yeast KSS1 and FUS3 genes. It is not essential for vegetative growth of Schizosaccharomyces pombe cells but is required for conjugation. The spk1+ gene product is a 45-kD protein enriched in the nucleus, and its level increases 10-fold after addition of staurosporine. The other gene pap1+ encodes an AP-1-like transcription factor that contains a region rich in basic amino acids followed by a "leucine zipper" motif. The pap1+ gene is required for spk1(+)-conferred staurosporine resistance. These two genes appear to function as a part of the fission yeast growth control pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / pharmacology*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basic-Leucine Zipper Transcription Factors
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Drug Resistance, Microbial / genetics*
  • Fungal Proteins*
  • Genes, Fungal
  • Immunohistochemistry
  • Molecular Sequence Data
  • Mutation
  • Pancreatitis-Associated Proteins
  • Phenotype
  • Plasmids
  • Protein Kinases / genetics*
  • Proto-Oncogene Proteins c-jun
  • Schizosaccharomyces / enzymology
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins
  • Sequence Homology, Nucleic Acid
  • Staurosporine
  • Transcription Factors / genetics*
  • Transcription, Genetic

Substances

  • Alkaloids
  • Basic-Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • Fungal Proteins
  • Pancreatitis-Associated Proteins
  • Pap1 protein, S pombe
  • Proto-Oncogene Proteins c-jun
  • REG3A protein, human
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • Protein Kinases
  • Staurosporine

Associated data

  • GENBANK/M31698
  • GENBANK/M31699
  • GENBANK/M61223
  • GENBANK/M61224
  • GENBANK/M61225
  • GENBANK/X57078
  • GENBANK/X57334
  • GENBANK/X60231
  • GENBANK/X60232
  • GENBANK/X60233