Abstract
Staurosporine, a potent inhibitor of protein kinase C, arrests fission yeast cell elongation specifically at a stage immediately after cell division. We isolated two genes, which, when carried on multicopy plasmids, confer drug resistance in fission yeast. One, spk1+, encodes a protein kinase highly similar (54% identity) to those encoded by the mammalian ERK1/MAP2 kinase and the budding yeast KSS1 and FUS3 genes. It is not essential for vegetative growth of Schizosaccharomyces pombe cells but is required for conjugation. The spk1+ gene product is a 45-kD protein enriched in the nucleus, and its level increases 10-fold after addition of staurosporine. The other gene pap1+ encodes an AP-1-like transcription factor that contains a region rich in basic amino acids followed by a "leucine zipper" motif. The pap1+ gene is required for spk1(+)-conferred staurosporine resistance. These two genes appear to function as a part of the fission yeast growth control pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / pharmacology*
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Amino Acid Sequence
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Animals
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Base Sequence
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Basic-Leucine Zipper Transcription Factors
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Chromosome Mapping
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Cloning, Molecular
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Drug Resistance, Microbial / genetics*
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Fungal Proteins*
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Genes, Fungal
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Immunohistochemistry
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Molecular Sequence Data
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Mutation
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Pancreatitis-Associated Proteins
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Phenotype
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Plasmids
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Protein Kinases / genetics*
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Proto-Oncogene Proteins c-jun
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Schizosaccharomyces / enzymology
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Schizosaccharomyces / genetics*
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Schizosaccharomyces pombe Proteins
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Sequence Homology, Nucleic Acid
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Staurosporine
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Transcription Factors / genetics*
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Transcription, Genetic
Substances
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Alkaloids
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Basic-Leucine Zipper Transcription Factors
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DNA-Binding Proteins
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Fungal Proteins
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Pancreatitis-Associated Proteins
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Pap1 protein, S pombe
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Proto-Oncogene Proteins c-jun
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REG3A protein, human
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Schizosaccharomyces pombe Proteins
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Transcription Factors
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Protein Kinases
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Staurosporine
Associated data
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GENBANK/M31698
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GENBANK/M31699
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GENBANK/M61223
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GENBANK/M61224
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GENBANK/M61225
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GENBANK/X57078
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GENBANK/X57334
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GENBANK/X60231
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GENBANK/X60232
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GENBANK/X60233