Abstract
The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of ultraviolet-damaged DNA, and it functions in mitotic recombination. RAD10 has homology to the human excision repair gene ERCC-1. Here we describe the purification of the protein encoded by RAD10 and show that it is a DNA-binding protein with a strong preference for single-stranded DNA. We also show that RAD10 promotes the renaturation of complementary DNA strands.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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DNA Repair*
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DNA, Fungal / metabolism*
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DNA, Single-Stranded / metabolism
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Endonucleases*
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Fungal Proteins / metabolism*
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Fungal Proteins / pharmacology
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Nucleic Acid Renaturation* / drug effects
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Recombination, Genetic
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Single-Strand Specific DNA and RNA Endonucleases / metabolism
Substances
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DNA, Fungal
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DNA, Single-Stranded
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Endonucleases
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RAD10 protein, S cerevisiae
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Single-Strand Specific DNA and RNA Endonucleases