Abstract
Ultraviolet irradiation of fission yeast cells in G1 phase induced a delay in chromatin binding of replication initiation factors and, consistently, a transient delay in S-phase entry. The cell cycle delay was totally dependent on the Gcn2 kinase, a sensor of the nutritional status, and was accompanied by phosphorylation of the translation initiation factor eIF2alpha and by a general depression of translation. However, the G1-specific synthesis of factors required for DNA replication was not reduced by ultraviolet radiation. The cell cycle delay represents a novel checkpoint with a novel mechanism of action that is not activated by ionizing radiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Cycle Proteins / metabolism
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Enzyme Activation / radiation effects
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Eukaryotic Initiation Factor-2 / genetics
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Eukaryotic Initiation Factor-2 / metabolism
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Eukaryotic Initiation Factor-2 / radiation effects
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G1 Phase / radiation effects
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Minichromosome Maintenance Complex Component 6
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Origin Recognition Complex / metabolism
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Phosphorylation / radiation effects
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Protein Biosynthesis / radiation effects
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Protein Serine-Threonine Kinases / metabolism
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S Phase / radiation effects
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Schizosaccharomyces / cytology*
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Schizosaccharomyces / genetics
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Schizosaccharomyces / metabolism
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Schizosaccharomyces / radiation effects
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Schizosaccharomyces pombe Proteins / metabolism
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Signal Transduction / radiation effects
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Ultraviolet Rays
Substances
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Cell Cycle Proteins
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Eukaryotic Initiation Factor-2
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Origin Recognition Complex
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Schizosaccharomyces pombe Proteins
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Protein Serine-Threonine Kinases
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Minichromosome Maintenance Complex Component 6
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mcm2 protein, S pombe
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mcm6 protein, S pombe