Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes

Yufuko Akamatsu; Yasuhiro Tsutsui; Takashi Morishita; Md Shahjahan P Siddique; Yumiko Kurokawa; Mitsunori Ikeguchi; Fumiaki Yamao; Benoit Arcangioli; Hiroshi Iwasaki

doi:10.1038/sj.emboj.7601582

Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes

EMBO J. 2007 Mar 7;26(5):1352-62. doi: 10.1038/sj.emboj.7601582. Epub 2007 Feb 15.

Authors

Yufuko Akamatsu¹, Yasuhiro Tsutsui, Takashi Morishita, Md Shahjahan P Siddique, Yumiko Kurokawa, Mitsunori Ikeguchi, Fumiaki Yamao, Benoit Arcangioli, Hiroshi Iwasaki

Affiliation

¹ International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, Kanagawa, Japan.

Abstract

Several accessory proteins referred to as mediators are required for the full activity of the Rad51 (Rhp51 in fission yeast) recombinase. In this study, we analyzed in vivo functions of the recently discovered Swi5/Sfr1 complex from fission yeast. In normally growing cells, the Swi5-GFP protein localizes to the nucleus, where it forms a diffuse nuclear staining pattern with a few distinct foci. These spontaneous foci do not form in swi2Delta mutants. Upon UV irradiation, Swi5 focus formation is induced in swi2Delta mutants, a response that depends on Sfr1 function, and Sfr1 also forms foci that colocalize with damage-induced Rhp51 foci. The number of UV-induced Rhp51 foci is partially reduced in swi5Delta and rhp57Delta mutants and completely abolished in an swi5Delta rhp57Delta double mutant. An assay for products generated by HO endonuclease-induced DNA double-strand breaks (DSBs) reveals that Rhp51 and Rhp57, but not Swi5/Sfr1, are essential for crossover production. These results suggest that Swi5/Sfr1 functions as an Rhp51 mediator but processes DSBs in a manner different from that of the Rhp55/57 mediator.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / metabolism
Cell Nucleus / metabolism
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism
DNA Breaks, Double-Stranded / radiation effects
DNA Damage
DNA Repair / genetics
DNA, Fungal / genetics
DNA, Fungal / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Heterochromatin / metabolism
Microscopy, Fluorescence
Protein Binding
Rad51 Recombinase / genetics
Rad51 Recombinase / metabolism
Recombination, Genetic*
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism*
Schizosaccharomyces / radiation effects
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism*
Ultraviolet Rays

Substances

Chromosomal Proteins, Non-Histone
DNA, Fungal
DNA-Binding Proteins
Heterochromatin
RHP51 protein, S pombe
RHP55 protein, S pombe
Schizosaccharomyces pombe Proteins
Sfr1 protein, S pombe
Swi5 protein, S pombe
Swi6 protein, S pombe
Green Fluorescent Proteins
Rad51 Recombinase
Adenosine Triphosphatases
Rhp57 protein, S pombe