Arl1p is involved in transport of the GPI-anchored protein Gas1p from the late Golgi to the plasma membrane

Ya-Wen Liu; Szu-Wei Lee; Fang-Jen S Lee

doi:10.1242/jcs.03148

Arl1p is involved in transport of the GPI-anchored protein Gas1p from the late Golgi to the plasma membrane

J Cell Sci. 2006 Sep 15;119(Pt 18):3845-55. doi: 10.1242/jcs.03148. Epub 2006 Aug 22.

Authors

Ya-Wen Liu¹, Szu-Wei Lee, Fang-Jen S Lee

Affiliation

¹ Institute of Molecular Medicine, School of Medicine, National Taiwan University, and Department of Medical Research, National Taiwan University Hospital, Taipei 100, Taiwan.

PMID: 16926193
DOI: 10.1242/jcs.03148

Abstract

The molecular mechanisms involved in the transport of GPI-anchored proteins from the trans-Golgi network (TGN) to the cell periphery have not been established. Arl1p is a member of the Arf-like protein (Arl) subfamily of small GTPases and is localized in the late Golgi. Although Arl1p is implicated in regulation of Golgi structure and function, no endogenous cargo protein that is regulated by Arl1p has been identified in yeast. In this study, we demonstrate that Arl1p is involved in the anterograde transport from the Golgi to the cell surface of the glycosylphosphatidylinositol (GPI)-anchored plasma-membrane-resident protein Gas1p, but not the cell-wall-localized GPI-anchored proteins Crh1p, Crh2p and Cwp1p, or non-GPI-anchored plasma membrane-protein Gap1p. We also show that regulators of Arl1p (Sys1p, Arl3p and Gcs1p) and an effector (Imh1p) all participate in the transport of Gas1p. Thus, we infer that the signaling cascade Sys1p-Arl3p-Arl1p-Imh1p specifically participates in the transport of a GPI-anchored protein from the late Golgi to the plasma membrane.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ADP-Ribosylation Factors / metabolism
Cell Membrane / metabolism*
Cell Wall / metabolism
Congo Red
Gene Deletion
Glycosylphosphatidylinositols / metabolism*
Golgi Apparatus / metabolism*
Membrane Glycoproteins / metabolism*
Membrane Proteins / metabolism
Monomeric GTP-Binding Proteins / metabolism*
Protein Transport
R-SNARE Proteins / metabolism
Recombinant Fusion Proteins / metabolism
Saccharomyces cerevisiae / cytology*
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Vacuoles / metabolism
Vesicular Transport Proteins / metabolism*

Substances

GAS1 protein, S cerevisiae
Glycosylphosphatidylinositols
IMH1 protein, S cerevisiae
Membrane Glycoproteins
Membrane Proteins
R-SNARE Proteins
Recombinant Fusion Proteins
SNC1 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Sys1 protein, S cerevisiae
Vesicular Transport Proteins
Congo Red
ARL1 protein, S cerevisiae
ADP-Ribosylation Factors
ARL3 protein, S cerevisiae
Monomeric GTP-Binding Proteins
YPT6 protein, S cerevisiae