Abstract
The MtArg8 reversion assay, which measures point mutation in mtDNA, indicates that in budding yeast Saccharomyces cerevisiae, DNA polymerase zeta and Rev1 proteins participate in the mitochondrial DNA mutagenesis. Supporting this evidence, both polymerase zeta and Rev1p were found to be localized in the mitochondria. This is the first report demonstrating that the DNA polymerase zeta and Rev1 proteins function in the mitochondria.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Mitochondrial / metabolism
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DNA-Directed DNA Polymerase / genetics*
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Epistasis, Genetic
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Green Fluorescent Proteins / metabolism
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Humans
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Microscopy, Fluorescence
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Mutation
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Nucleotidyltransferases / genetics*
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Point Mutation*
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Recombinant Fusion Proteins / chemistry
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins / genetics*
Substances
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DNA, Mitochondrial
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REV7 protein, S cerevisiae
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins
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Nucleotidyltransferases
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REV1 protein, S cerevisiae
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DNA-Directed DNA Polymerase
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REV3 protein, S cerevisiae