The fission yeast gene encoding monothiol glutaredoxin 5 is regulated by nitrosative and osmotic stresses

Mol Cells. 2005 Aug 31;20(1):43-50.

Abstract

Glutaredoxin (Grx) is a small, heat-stable redox protein acting as a multi-functional glutathione (GSH)-dependent disulfide oxidoreductase. We have cloned the monothiol Grx5 gene from the genomic DNA of the fission yeast Schizosaccharomyces pombe. It has 1,904 bp, with one intron, and encodes a putative protein of 146 amino acids with a molecular mass of 16.5 kDa. Recombinant Grx5 produced functional Grx in S. pombe cells. NO-generating sodium nitroprusside (SNP, 1.0 and 2.0 mM) and potassium chloride (KCl, 0.2 and 0.5 M) increased the synthesis of beta-galactosidase from a Grx5-lacZ fusion gene, and transcription of Grx5 was also enhanced by SNP and KCl. Synthesis of beta-galactosidase from the Grx5-lacZ fusion was lower in Pap1-negative TP108-3C cells than in wild type KP1 cells, and when Pap1 was overproduced in KP1 cells, the level of beta-galactosidase increased. We also found that Pap1 is involved in the induction of Grx5 by SNP and KCl. S. pombe Grx5 may play a crucial role in responses to nitrosative and osmotic stresses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Basic-Leucine Zipper Transcription Factors / metabolism
  • Basic-Leucine Zipper Transcription Factors / pharmacology
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation*
  • Glutaredoxins
  • Molecular Sequence Data
  • Nitroprusside / metabolism*
  • Nitroprusside / pharmacology
  • Osmotic Pressure*
  • Oxidative Stress / genetics
  • Oxidative Stress / physiology
  • Oxidoreductases / genetics*
  • Oxidoreductases / metabolism
  • Oxidoreductases / physiology
  • Pancreatitis-Associated Proteins
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reactive Oxygen Species / metabolism
  • Reactive Oxygen Species / pharmacology
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism
  • Schizosaccharomyces / physiology
  • Schizosaccharomyces pombe Proteins / metabolism
  • Schizosaccharomyces pombe Proteins / pharmacology
  • Sequence Alignment
  • Time Factors
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Basic-Leucine Zipper Transcription Factors
  • Glutaredoxins
  • Pancreatitis-Associated Proteins
  • Pap1 protein, S pombe
  • REG3A protein, human
  • RNA, Messenger
  • Reactive Oxygen Species
  • Schizosaccharomyces pombe Proteins
  • Nitroprusside
  • Oxidoreductases
  • beta-Galactosidase