Abstract
Recombination-mediated pathways for telomere lengthening may be utilized in the absence of telomerase activity. The RecQ-like helicases, BLM and Sgs1, are implicated in recombination-mediated telomere lengthening in human cells and budding yeast, respectively. Here, we show that BLM expression rescues disrupted telomere lengthening in telomerase-negative sgs1 yeast. BLM helicase activity is required for this complementation, indicating BLM and Sgs1 resolve the same telomeric structures. These data support a conserved function for BLM and Sgs1 in recombination-mediated telomere lengthening.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism
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Adenosine Triphosphatases / physiology*
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DNA Helicases / deficiency
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DNA Helicases / genetics
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DNA Helicases / metabolism
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DNA Helicases / physiology*
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Mutagenesis, Site-Directed
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RecQ Helicases
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / ultrastructure
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Saccharomyces cerevisiae Proteins / physiology*
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Telomerase / deficiency*
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Telomere / genetics
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Telomere / metabolism
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Telomere / physiology*
Substances
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Saccharomyces cerevisiae Proteins
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Telomerase
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Adenosine Triphosphatases
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Bloom syndrome protein
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SGS1 protein, S cerevisiae
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DNA Helicases
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RecQ Helicases