Osmotin is a homolog of mammalian adiponectin and controls apoptosis in yeast through a homolog of mammalian adiponectin receptor

Meena L Narasimhan; María A Coca; Jingbo Jin; Toshimasa Yamauchi; Yusuke Ito; Takashi Kadowaki; Kyeong Kyu Kim; José M Pardo; Barbara Damsz; Paul M Hasegawa; Dae-Jin Yun; Ray A Bressan

doi:10.1016/j.molcel.2004.11.050

Osmotin is a homolog of mammalian adiponectin and controls apoptosis in yeast through a homolog of mammalian adiponectin receptor

Mol Cell. 2005 Jan 21;17(2):171-80. doi: 10.1016/j.molcel.2004.11.050.

Authors

Meena L Narasimhan¹, María A Coca, Jingbo Jin, Toshimasa Yamauchi, Yusuke Ito, Takashi Kadowaki, Kyeong Kyu Kim, José M Pardo, Barbara Damsz, Paul M Hasegawa, Dae-Jin Yun, Ray A Bressan

Affiliation

¹ Center for Plant Environmental Stress Physiology, Purdue University, 625 Agriculture Mall Drive, West Lafayette, Indiana [corrected] 47907, USA.

PMID: 15664187
DOI: 10.1016/j.molcel.2004.11.050

Abstract

The antifungal activity of the PR-5 family of plant defense proteins has been suspected to involve specific plasma membrane component(s) of the fungal target. Osmotin is a tobacco PR-5 family protein that induces apoptosis in the yeast Saccharomyces cerevisiae. We show here that the protein encoded by ORE20/PHO36 (YOL002c), a seven transmembrane domain receptor-like polypeptide that regulates lipid and phosphate metabolism, is an osmotin binding plasma membrane protein that is required for full sensitivity to osmotin. PHO36 functions upstream of RAS2 in the osmotin-induced apoptotic pathway. The mammalian homolog of PHO36 is a receptor for the hormone adiponectin and regulates cellular lipid and sugar metabolism. Osmotin and adiponectin, the corresponding "receptor" binding proteins, do not share sequence similarity. However, the beta barrel domain of both proteins can be overlapped, and osmotin, like adiponectin, activates AMP kinase in C2C12 myocytes via adiponectin receptors.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Adiponectin
Amino Acid Sequence
Animals
Antifungal Agents / metabolism
Apoptosis*
Base Sequence
Intercellular Signaling Peptides and Proteins / chemistry
Intercellular Signaling Peptides and Proteins / genetics
Intercellular Signaling Peptides and Proteins / metabolism*
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Models, Molecular
Molecular Sequence Data
Phenotype
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / metabolism*
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
Saccharomyces cerevisiae / physiology*
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*
Signal Transduction / physiology
ras Proteins / metabolism

Substances

Adiponectin
Antifungal Agents
IZH2 protein, S cerevisiae
Intercellular Signaling Peptides and Proteins
Membrane Proteins
Plant Proteins
Protein Isoforms
RNA, Small Interfering
Saccharomyces cerevisiae Proteins
RAS2 protein, S cerevisiae
ras Proteins