Abstract
Among the few proteins of the eukaryotic nucleolus that have been characterized, four proteins, nucleolin, fibrillarin, SSB1 and NSR1, possess a common structural motif, the GAR domain, which is rich in glycine and arginine residues. In order to examine whether the presence of this domain is characteristic of a family of nucleolar proteins, we investigated whether other yeast genes encode proteins containing GAR domains. We report here the sequence and the characterization of a new yeast gene, GAR1, which encodes a protein of 205 residues containing two GAR domains. GAR1 is a non-ribosomal protein, localized in the yeast nucleolus, which is essential for cell growth. Immunoprecipitation with anti-GAR1 antibodies shows that GAR1 is associated with a subset of snoRNAs, including snR10 and snR30. Depletion of GAR1 by expression under the control of a regulated GAL promoter, impairs processing of the 35S primary transcript of pre-rRNA and prevents synthesis of 18S rRNA. GAR1 is thus the fifth member of a family of nucleolar proteins containing GAR domains, and is involved in rRNA metabolism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Southern
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Cloning, Molecular
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DNA, Fungal / genetics
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DNA, Fungal / isolation & purification
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Escherichia coli / genetics
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Fungal Proteins / genetics*
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Genes, Fungal*
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Molecular Sequence Data
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Multigene Family
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Nuclear Proteins / genetics*
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RNA Precursors / genetics*
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RNA Processing, Post-Transcriptional*
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Recombinant Fusion Proteins / metabolism
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Restriction Mapping
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Ribonucleoproteins / genetics*
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Ribonucleoproteins / metabolism*
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Ribonucleoproteins, Small Nuclear
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Ribonucleoproteins, Small Nucleolar*
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Ribosomes / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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DNA, Fungal
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Fungal Proteins
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Nuclear Proteins
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RNA Precursors
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Recombinant Fusion Proteins
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Ribonucleoproteins
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Ribonucleoproteins, Small Nuclear
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Ribonucleoproteins, Small Nucleolar
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Saccharomyces cerevisiae Proteins
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GAR1 protein, S cerevisiae
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beta-Galactosidase
Associated data
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GENBANK/D13200
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GENBANK/D13201
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GENBANK/D13202
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GENBANK/D13203
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GENBANK/L07339
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GENBANK/M94783
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GENBANK/M94784
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GENBANK/M94785
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GENBANK/X63350
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GENBANK/X63617