Abstract
The small GTPase Arf and coatomer (COPI) are required for the generation of retrograde transport vesicles. Arf activity is regulated by guanine exchange factors (ArfGEF) and GTPase-activating proteins (ArfGAPs). The ArfGAPs Gcs1 and Glo3 provide essential overlapping function for retrograde vesicular transport from the Golgi to the endoplasmic reticulum. We have identified Glo3 as a component of COPI vesicles. Furthermore, we find that a mutant version of the Glo3 protein exerts a negative effect on retrograde transport, even in the presence of the ArfGAP Gcs1. Finally, we present evidence supporting a role for ArfGAP protein in the generation of COPI retrograde transport vesicles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP-Ribosylation Factors / genetics
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ADP-Ribosylation Factors / metabolism*
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Base Sequence
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Biological Transport, Active
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COP-Coated Vesicles / metabolism*
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DNA, Fungal / genetics
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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GTPase-Activating Proteins / genetics
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GTPase-Activating Proteins / metabolism*
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Genes, Fungal
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Microscopy, Electron
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Mutagenesis, Site-Directed
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Mutation
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Vesicular Transport Proteins
Substances
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DNA, Fungal
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DNA-Binding Proteins
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EMP47 protein, S cerevisiae
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GCS1 protein, S cerevisiae
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GTPase-Activating Proteins
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Membrane Proteins
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Saccharomyces cerevisiae Proteins
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Vesicular Transport Proteins
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ADP-Ribosylation Factors