The mitochondrial morphology protein Mdm10 functions in assembly of the preprotein translocase of the outer membrane

Chris Meisinger; Michael Rissler; Agnieszka Chacinska; Luiza K Sanjuán Szklarz; Dusanka Milenkovic; Vera Kozjak; Birgit Schönfisch; Christiane Lohaus; Helmut E Meyer; Michael P Yaffe; Bernard Guiard; Nils Wiedemann; Nikolaus Pfanner

doi:10.1016/j.devcel.2004.06.003

The mitochondrial morphology protein Mdm10 functions in assembly of the preprotein translocase of the outer membrane

Dev Cell. 2004 Jul;7(1):61-71. doi: 10.1016/j.devcel.2004.06.003.

Authors

Chris Meisinger¹, Michael Rissler, Agnieszka Chacinska, Luiza K Sanjuán Szklarz, Dusanka Milenkovic, Vera Kozjak, Birgit Schönfisch, Christiane Lohaus, Helmut E Meyer, Michael P Yaffe, Bernard Guiard, Nils Wiedemann, Nikolaus Pfanner

Affiliation

¹ Institut für Biochemie und Molekularbiologie, Universität Freiburg, Hermann-Herder-Strasse 7, D-79104 Freiburg, Germany.

PMID: 15239954
DOI: 10.1016/j.devcel.2004.06.003

Abstract

The biogenesis of mitochondrial outer membrane proteins involves the general translocase of the outer membrane (TOM complex) and the sorting and assembly machinery (SAM complex). The two known subunits of the SAM complex, Mas37 and Sam50, are required for assembly of the abundant outer membrane proteins porin and Tom40. We have identified an unexpected subunit of the SAM complex, Mdm10, which is involved in maintenance of mitochondrial morphology. Mitochondria lacking Mdm10 are selectively impaired in the final steps of the assembly pathway of Tom40, including the association of Tom40 with the receptor Tom22 and small Tom proteins, while the biogenesis of porin is not affected. Yeast mutants of TOM40, MAS37, and SAM50 also show aberrant mitochondrial morphology. We conclude that Mdm10 plays a specific role in the biogenesis of the TOM complex, indicating a connection between the mitochondrial protein assembly apparatus and the machinery for maintenance of mitochondrial morphology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
Intracellular Membranes / enzymology
Intracellular Membranes / metabolism*
Intracellular Membranes / ultrastructure
Macromolecular Substances
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Membrane Transport Proteins / biosynthesis
Membrane Transport Proteins / genetics
Membrane Transport Proteins / metabolism
Mitochondria / metabolism*
Mitochondria / pathology
Mitochondria / ultrastructure
Mitochondrial Membrane Transport Proteins / biosynthesis*
Mitochondrial Membrane Transport Proteins / genetics
Mitochondrial Proteins / genetics
Mitochondrial Proteins / metabolism
Mutation / genetics
Protein Transport / genetics
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae / ultrastructure
Saccharomyces cerevisiae Proteins / biosynthesis
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*

Substances

MDM10 protein, S cerevisiae
Macromolecular Substances
Membrane Proteins
Membrane Transport Proteins
Mitochondrial Membrane Transport Proteins
Mitochondrial Proteins
SAM37 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Sam50 protein, S cerevisiae
TOM22 protein, S cerevisiae
Tom40 protein, S cerevisiae