Genome-wide analysis of ARS (autonomously replicating sequence) binding factor 1 (Abf1p)-mediated transcriptional regulation in Saccharomyces cerevisiae

J Biol Chem. 2004 Aug 13;279(33):34865-72. doi: 10.1074/jbc.M405156200. Epub 2004 Jun 10.

Abstract

Autonomously replicating sequence-binding factor-1 (Abf1p) is an essential sequence-specific transcription factor in Saccharomyces cerevisiae that participates in multiple nuclear events including DNA replication, transcription activation, and gene silencing. Numerous gene-specific analyses have implicated Abf1p in the transcriptional control of genes involved in a diverse range of cellular functions, leading to the notion that Abf1p acts as a global transcriptional regulator. Here we report findings from a genome-wide comparison of the gene expression profiles in the wild-type and abf1-1 temperature-sensitive mutant. The study identifies a total of 86 Abf1p-regulated genes (1.4% of the genome) of which 50 are activated and 36 are repressed by Abf1p. Interestingly, Abf1p binds to its own promoter in vivo and strongly represses its own transcription, suggesting a potential negative regulatory loop in Abf1p-mediated gene regulation. A comparison of our microarray data with the available databases reveals a significant overlap of genes regulated by Abf1p and those by several general transcription factors such as Mot1p and TAFs (TATA-binding protein-associated factors). Different mutant alleles of abf1 affect Abf1p-mediated transcription in a gene-dependent manner. Furthermore, Abf1p in vivo is associated with the promoter region of most Abf1p-activated but not with that of most Abf1p-repressed genes. Taken together, these results strongly suggest distinct underlying mechanisms by which Abf1p regulates gene expression.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 5' Untranslated Regions
  • Alleles
  • Blotting, Northern
  • Chromatin / metabolism
  • DNA-Binding Proteins* / genetics*
  • Gene Expression Regulation, Fungal*
  • Gene Silencing
  • Genome, Fungal*
  • Molecular Sequence Data
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Plasmids / metabolism
  • Precipitin Tests
  • Promoter Regions, Genetic
  • RNA / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins* / genetics*
  • Temperature
  • Transcription Factors* / genetics*
  • Transcription, Genetic*
  • Transcriptional Activation

Substances

  • 5' Untranslated Regions
  • ABF1 protein, S cerevisiae
  • Chromatin
  • DNA-Binding Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • RNA

Associated data

  • GENBANK/GSM25879
  • GENBANK/GSM25880
  • GENBANK/GSM25881
  • GENBANK/GSM25882