A new class of C. elegans synMuv genes implicates a Tip60/NuA4-like HAT complex as a negative regulator of Ras signaling

Dev Cell. 2004 Apr;6(4):563-76. doi: 10.1016/s1534-5807(04)00065-6.

Abstract

The class A and class B synMuv genes are functionally redundant negative regulators of a Ras signaling pathway that induces C. elegans vulval development. A number of class B synMuv genes encode components of an Rb and histone deacetylase complex that likely acts to repress transcription of genes required for vulval induction. We discovered a new class of synMuv genes that acts redundantly with both the A and B classes of genes in vulval cell-fate determination. These new class C synMuv genes encode TRRAP, MYST family histone acetyltransferase, and Enhancer of Polycomb homologs, which form a putative C. elegans Tip60/NuA4-like histone acetyltransferase complex. A fourth gene with partial class C synMuv properties encodes a homolog of the mammalian SWI/SNF family ATPase p400. Our findings indicate that the coordinated action of two chromatin-modifying complexes, one with histone deacetylase and the other with histone acetyltransferase activity, is important in regulating Ras signaling and specifying cell fates during C. elegans development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyltransferases / genetics*
  • Acetyltransferases / isolation & purification
  • Acetyltransferases / metabolism*
  • Adaptor Proteins, Signal Transducing
  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / isolation & purification
  • Adenosine Triphosphatases / metabolism*
  • Animals
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / growth & development*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / isolation & purification*
  • Caenorhabditis elegans Proteins / metabolism*
  • Cell Lineage / genetics
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / isolation & purification
  • Chromosomal Proteins, Non-Histone / metabolism*
  • DNA, Complementary / analysis
  • DNA, Complementary / genetics
  • Female
  • Gene Expression Regulation, Developmental / genetics*
  • Histone Acetyltransferases
  • Histone Deacetylases / genetics
  • Histone Deacetylases / isolation & purification
  • Lysine Acetyltransferase 5
  • Macromolecular Substances
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Molecular Sequence Data
  • Mutation / genetics
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Repressor Proteins
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Trans-Activators / genetics
  • Trans-Activators / isolation & purification
  • Trans-Activators / metabolism*
  • Transcription Factors
  • Vulva / cytology
  • Vulva / growth & development
  • Vulva / metabolism
  • ras Proteins / genetics
  • ras Proteins / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • Caenorhabditis elegans Proteins
  • Chromosomal Proteins, Non-Histone
  • DNA, Complementary
  • EPC-1 protein, C elegans
  • EPC1 protein, human
  • Macromolecular Substances
  • Membrane Glycoproteins
  • Nuclear Proteins
  • Repressor Proteins
  • TRR-1 protein, C elegans
  • Trans-Activators
  • Transcription Factors
  • transformation-transcription domain-associated protein
  • Acetyltransferases
  • Histone Acetyltransferases
  • KAT5 protein, human
  • Lysine Acetyltransferase 5
  • MYS-1 protein, C elegans
  • Histone Deacetylases
  • Adenosine Triphosphatases
  • SSL-1 protein, C elegans
  • ras Proteins

Associated data

  • GENBANK/AY551963
  • GENBANK/AY551964
  • GENBANK/AY551965
  • GENBANK/AY551966