Abstract
The class A and class B synMuv genes are functionally redundant negative regulators of a Ras signaling pathway that induces C. elegans vulval development. A number of class B synMuv genes encode components of an Rb and histone deacetylase complex that likely acts to repress transcription of genes required for vulval induction. We discovered a new class of synMuv genes that acts redundantly with both the A and B classes of genes in vulval cell-fate determination. These new class C synMuv genes encode TRRAP, MYST family histone acetyltransferase, and Enhancer of Polycomb homologs, which form a putative C. elegans Tip60/NuA4-like histone acetyltransferase complex. A fourth gene with partial class C synMuv properties encodes a homolog of the mammalian SWI/SNF family ATPase p400. Our findings indicate that the coordinated action of two chromatin-modifying complexes, one with histone deacetylase and the other with histone acetyltransferase activity, is important in regulating Ras signaling and specifying cell fates during C. elegans development.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / genetics*
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Acetyltransferases / isolation & purification
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Acetyltransferases / metabolism*
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Adaptor Proteins, Signal Transducing
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / isolation & purification
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Adenosine Triphosphatases / metabolism*
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Animals
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / growth & development*
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / isolation & purification*
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Caenorhabditis elegans Proteins / metabolism*
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Cell Lineage / genetics
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / isolation & purification
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Chromosomal Proteins, Non-Histone / metabolism*
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DNA, Complementary / analysis
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DNA, Complementary / genetics
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Female
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Gene Expression Regulation, Developmental / genetics*
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Histone Acetyltransferases
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Histone Deacetylases / genetics
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Histone Deacetylases / isolation & purification
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Lysine Acetyltransferase 5
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Macromolecular Substances
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism
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Molecular Sequence Data
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Mutation / genetics
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Repressor Proteins
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Trans-Activators / genetics
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Trans-Activators / isolation & purification
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Trans-Activators / metabolism*
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Transcription Factors
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Vulva / cytology
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Vulva / growth & development
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Vulva / metabolism
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ras Proteins / genetics
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ras Proteins / metabolism*
Substances
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Adaptor Proteins, Signal Transducing
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Caenorhabditis elegans Proteins
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Chromosomal Proteins, Non-Histone
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DNA, Complementary
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EPC-1 protein, C elegans
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EPC1 protein, human
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Macromolecular Substances
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Membrane Glycoproteins
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Nuclear Proteins
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Repressor Proteins
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TRR-1 protein, C elegans
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Trans-Activators
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Transcription Factors
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transformation-transcription domain-associated protein
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Acetyltransferases
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Histone Acetyltransferases
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KAT5 protein, human
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Lysine Acetyltransferase 5
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MYS-1 protein, C elegans
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Histone Deacetylases
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Adenosine Triphosphatases
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SSL-1 protein, C elegans
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ras Proteins
Associated data
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GENBANK/AY551963
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GENBANK/AY551964
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GENBANK/AY551965
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GENBANK/AY551966