An NDPase links ADAM protease glycosylation with organ morphogenesis in C. elegans

Kiyoji Nishiwaki; Yukihiko Kubota; Yuko Chigira; Samir Kumar Roy; Maho Suzuki; Mara Schvarzstein; Yoshifumi Jigami; Naoki Hisamoto; Kunihiro Matsumoto

doi:10.1038/ncb1079

An NDPase links ADAM protease glycosylation with organ morphogenesis in C. elegans

Nat Cell Biol. 2004 Jan;6(1):31-7. doi: 10.1038/ncb1079. Epub 2003 Dec 21.

Authors

Kiyoji Nishiwaki¹, Yukihiko Kubota, Yuko Chigira, Samir Kumar Roy, Maho Suzuki, Mara Schvarzstein, Yoshifumi Jigami, Naoki Hisamoto, Kunihiro Matsumoto

Affiliation

¹ RIKEN Center for Developmental Biology and PRESTO, Japan Science and Technology Corporation, Chuo-ku, Kobe 650-0047, Japan. nishiwak@cdb.riken.go.jp

PMID: 14688791
DOI: 10.1038/ncb1079

Abstract

In the nematode Caenorhabditis elegans, the gonad acquires two U-shaped arms through the directed migration of its distal tip cells (DTCs), which are located at the tip of the growing gonad arms. A member of the ADAM (a disintegrin and metalloprotease) family, MIG-17, regulates directional migration of DTCs: MIG-17 is synthesized and secreted from the muscle cells of the body wall, and diffuses to the gonad where it is required for DTC migration. The mig-23 mutation causes defective migration of DTCs and interacts genetically with mig-17. Here, we report that mig-23 encodes a membrane-bound nucleoside diphosphatase (NDPase) required for glycosylation and proper localization of MIG-17. Our findings indicate that an NDPase affects organ morphogenesis through glycosylation of the MIG-17 ADAM protease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acid Anhydride Hydrolases / genetics
Acid Anhydride Hydrolases / isolation & purification*
Acid Anhydride Hydrolases / metabolism*
Animals
Animals, Genetically Modified
Base Sequence / genetics
Caenorhabditis elegans / cytology
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / enzymology
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / isolation & purification
Caenorhabditis elegans Proteins / metabolism*
Cell Differentiation / genetics
Cell Lineage / genetics
Cell Movement / physiology
DNA, Complementary / analysis
DNA, Complementary / genetics
Disintegrins / metabolism*
Glycoproteins / metabolism
Glycosylation
Gonads / cytology
Gonads / embryology*
Gonads / metabolism
Metalloendopeptidases / metabolism*
Molecular Sequence Data
Muscle Cells / metabolism
Mutation / genetics
Organogenesis / physiology*
Stem Cells / cytology
Stem Cells / metabolism

Substances

Caenorhabditis elegans Proteins
DNA, Complementary
Disintegrins
Glycoproteins
MIG-17 protein, C elegans
Metalloendopeptidases
Acid Anhydride Hydrolases
MIG-23 protein, C elegans
nucleoside-diphosphatase

Associated data

GENBANK/AB126261