Abstract
In yeast Saccharomyces cerevisiae the G protein betagamma subunits (Ste4/Ste18) have long been known to transmit the signal required for mating. Here we demonstrate that GTPase-deficient mutants of Galpha (Gpa1) directly activate the mating response pathway. We also show that signaling by activated Gpa1 requires direct coupling to an RNA binding protein Scp160. These findings suggest an additional role for Gpa1 and reveal Scp160 as a component of the mating response pathway in yeast.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Dose-Response Relationship, Drug
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GTP-Binding Protein alpha Subunits / metabolism*
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GTP-Binding Protein alpha Subunits / physiology*
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GTP-Binding Protein alpha Subunits, Gq-G11
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Galactose / metabolism
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Genes, Fungal
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Glucose / metabolism
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Glutathione Transferase / metabolism
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Membrane Proteins / metabolism*
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Nuclear Proteins / metabolism*
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Oligonucleotide Array Sequence Analysis
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Plasmids / metabolism
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Protein Conformation
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RNA / metabolism*
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RNA-Binding Proteins
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins / metabolism*
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Saccharomyces cerevisiae Proteins / physiology*
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Signal Transduction
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Two-Hybrid System Techniques
Substances
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GTP-Binding Protein alpha Subunits
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Membrane Proteins
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Nuclear Proteins
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RNA-Binding Proteins
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SCP160 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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RNA
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Glutathione Transferase
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GPA1 protein, S cerevisiae
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GTP-Binding Protein alpha Subunits, Gq-G11
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Glucose
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Galactose