Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis

Curr Genet. 2003 Dec;44(4):184-94. doi: 10.1007/s00294-003-0442-z. Epub 2003 Sep 13.

Abstract

Saccharomyces cerevisiae ATS1 (alpha-tubulin suppressor 1) was originally identified as a high-copy suppressor of class two alpha-tubulin mutations and was proposed to have a regulatory role in coordinating the microtubule state with the cell cycle. Here, we show that Ats1p interacts with Nap1p, a cytoplasmic protein that regulates the activity of the Cdc28p/Clb2p complex. Loss of Nap1p results in a delayed switch from polar to isotropic bud growth. The delayed switch results in elongated buds. Nap1p and Ats1p interact in two-hybrid and co-immunoprecipitation assays. Both nap1Delta and ats1Delta cells have a Clb2p-dependent elongated bud morphology. Deletion of ATS1 partially suppresses the elongated bud morphology and benomyl resistance of nap1Delta mutants. Our results suggest Ats1p might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Benomyl
  • Blotting, Southern
  • Cell Cycle / physiology*
  • Cell Cycle Proteins
  • Chromosome Deletion
  • Drug Resistance, Fungal / genetics
  • Drug Resistance, Fungal / physiology
  • Microscopy, Confocal
  • Microtubules / physiology*
  • Nuclear Proteins
  • Nucleosome Assembly Protein 1
  • Oligonucleotides
  • Plasmids / genetics
  • Plasmids / metabolism
  • Precipitin Tests
  • Proteins / metabolism*
  • Repressor Proteins / metabolism*
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Two-Hybrid System Techniques

Substances

  • ATS1 protein, S cerevisiae
  • Cell Cycle Proteins
  • NAP1 protein, S cerevisiae
  • Nuclear Proteins
  • Nucleosome Assembly Protein 1
  • Oligonucleotides
  • Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Benomyl