Abstract
Eukaryotic RNA polymerase II transcribes precursors of mRNAs and of non-protein-coding RNAs such as snRNAs and snoRNAs. These RNAs have to be processed at their 3' ends to be functional. mRNAs are matured by cleavage and polyadenylation that require a well-characterized protein complex. Small RNAs are also subject to 3' end cleavage but are not polyadenylated. Here we show that two newly identified proteins, Pti1p and Ref2p, although they were found associated with the pre-mRNA 3' end processing complex, are essential for yeast snoRNA 3' end maturation. We also provide evidence that Pti1p probably acts by uncoupling cleavage and polyadenylation, and functions in coordination with the Nrd1p-dependent pathway for 3' end formation of non-polyadenylated transcripts.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Genes, Fungal
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Mutation
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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RNA Processing, Post-Transcriptional
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RNA, Fungal / genetics
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RNA, Fungal / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism*
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RNA, Small Nucleolar / genetics
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RNA, Small Nucleolar / metabolism*
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RNA-Binding Proteins
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Ribonucleoproteins / genetics
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Ribonucleoproteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Suppression, Genetic
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Temperature
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mRNA Cleavage and Polyadenylation Factors / genetics
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mRNA Cleavage and Polyadenylation Factors / metabolism
Substances
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Fungal Proteins
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NRD1 protein, S cerevisiae
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PTI1 protein, S cerevisiae
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RNA, Fungal
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RNA, Messenger
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RNA, Small Nucleolar
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RNA-Binding Proteins
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Ribonucleoproteins
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Saccharomyces cerevisiae Proteins
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mRNA Cleavage and Polyadenylation Factors
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Protein Serine-Threonine Kinases