Abstract
The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at the kinetochore in chromosome transmission. Mutations in genes encoding two core subunits of RSC, the ATPase Sth1p and the Snf5p homolog Sfh1p, interact genetically with mutations in genes encoding kinetochore proteins and with a mutation in centromeric DNA. RSC also interacts genetically and physically with the histone and histone variant components of centromeric chromatin. Importantly, RSC is localized to centromeric and centromere-proximal chromosomal regions, and its association with these loci is dependent on Sth1p. Both sth1 and sfh1 mutants exhibit altered centromeric and centromere-proximal chromatin structure and increased missegregation of authentic chromosomes. Finally, RSC is not required for centromeric deposition of the histone H3 variant Cse4p, suggesting that RSC plays a role in reconfiguring centromeric and flanking nucleosomes following Cse4p recruitment for proper chromosome transmission.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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Centromere / chemistry
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Centromere / physiology
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Chromatin / genetics
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Chromatin / metabolism*
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Chromatin / ultrastructure
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Chromosomal Proteins, Non-Histone
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Chromosome Segregation*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Histones / genetics
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Kinetochores / physiology*
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Macromolecular Substances
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Mutation
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Phospholipid Transfer Proteins
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Spindle Apparatus
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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CSE4 protein, S cerevisiae
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Carrier Proteins
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Cell Cycle Proteins
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Histones
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MIF2 protein, S cerevisiae
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Macromolecular Substances
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Membrane Proteins
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Nuclear Proteins
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Phospholipid Transfer Proteins
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RSC complex, S cerevisiae
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SEC24 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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STH1 protein, S cerevisiae