Abstract
To determine the function of Dna2 in a multicellular organism, the Caenorhabditis elegans Dna2 expression was probed and deletion mutant phenotypes were analyzed. Dna2 was localized to the nuclei of C. elegans oocytes and early embryos by immunostaining or green fluorescent protein-tagging. A homozygous dna2 deletion mutant showed a reduced brood size and embryonic lethality, and the phenotypes greatly depended on growth temperature and aggravated in the succeeding generation. The mutant embryos also showed delayed cell divisions, which together with temperature-dependence of the mutant phenotypes supported the well-conserved role of Dna2 in DNA replication.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / physiology*
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Amino Acid Sequence
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Animals
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Anticipation, Genetic
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Caenorhabditis elegans / embryology
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / physiology*
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / physiology*
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Cell Division
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DNA Helicases / genetics
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DNA Helicases / physiology*
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DNA Replication
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DNA, Helminth / biosynthesis
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DNA, Helminth / genetics
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Fertility / genetics
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Gene Expression Regulation, Developmental
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Genes, Lethal
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Genes, Reporter
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Green Fluorescent Proteins
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Luminescent Proteins / analysis
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Luminescent Proteins / genetics
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Molecular Sequence Data
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Oocytes / metabolism
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RNA Interference
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Temperature
Substances
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Caenorhabditis elegans Proteins
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DNA, Helminth
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Luminescent Proteins
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins
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Adenosine Triphosphatases
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DNA Helicases
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DNA2 protein, S cerevisiae