The yeast model for Batten disease: a role for Btn2p in the trafficking of the Golgi-associated vesicular targeting protein, Yif1p

Biochem Biophys Res Commun. 2003 Mar 14;302(3):534-8. doi: 10.1016/s0006-291x(03)00209-2.

Abstract

Btn2p is a novel coiled coil cytosolic protein in Saccharomyces cerevisiae. We report that Btn2p interacts with Yif1p, a component of a protein complex at the Golgi that functions in ER to Golgi transport. Deletion of Btn2p, btn2-delta, results in mis-localiztion of Yif1p to the vacuole. Therefore, Btn2p may have an apparent role in intracellular trafficking of proteins. Btn2p was originally identified as being up-regulated in a btn1-delta strain, which exhibits dysregulation of vacuolar pH, and this up-regulation of Btn2p was presumed to contribute to maintaining a stable vacuolar pH [Pearce et al. Nat. Genet. 22 (1999) 55]. We propose that up-regulation of Btn2p in btn1-delta is an indicator of altered trafficking within the cell, and as btn1-delta serves as a model for the lysosomal storage disorder Batten disease, that altered intracellular trafficking may contribute to some of the cellular pathological hallmarks of this disease.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Vesicular Transport
  • Amino Acid Transport Systems
  • Cyclins / genetics*
  • Cytosol / metabolism
  • Endoplasmic Reticulum / metabolism
  • Gene Deletion
  • Gene Library
  • Golgi Apparatus / metabolism*
  • Humans
  • Hydrogen-Ion Concentration
  • Membrane Glycoproteins*
  • Molecular Chaperones*
  • Neuronal Ceroid-Lipofuscinoses / genetics*
  • Neuronal Ceroid-Lipofuscinoses / metabolism
  • Neurons / metabolism
  • Phenotype
  • Precipitin Tests
  • Protein Binding
  • Protein Transport
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Saccharomyces cerevisiae Proteins / physiology*
  • Two-Hybrid System Techniques

Substances

  • Adaptor Proteins, Vesicular Transport
  • Amino Acid Transport Systems
  • BTN2 protein, S cerevisiae
  • CLN3 protein, S cerevisiae
  • CLN3 protein, human
  • Cyclins
  • Membrane Glycoproteins
  • Molecular Chaperones
  • Saccharomyces cerevisiae Proteins
  • YHC3 protein, S cerevisiae
  • YIF1 protein, S cerevisiae