Abstract
The Snf1 protein kinase of Saccharomyces cerevisiae has been shown to have a role in regulating haploid invasive growth in response to glucose depletion. Cells contain three forms of the Snf1 kinase, each with a different beta-subunit isoform, either Gal83, Sip1, or Sip2. We present evidence that different Snf1 kinases play distinct roles in two aspects of invasive growth, namely, adherence to the agar substrate and filamentation. The Snf1-Gal83 form of the kinase is required for adherence, whereas either Snf1-Gal83 or Snf1-Sip2 is sufficient for filamentation. Genetic evidence indicates that Snf1-Gal83 affects adherence by antagonizing Nrg1- and Nrg2-mediated repression of the FLO11 flocculin and adhesin gene. In contrast, the mechanism(s) by which Snf1-Gal83 and Snf1-Sip2 affect filamentation is independent of FLO11. Thus, the Snf1 kinase regulates invasive growth by at least two distinct mechanisms.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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AMP-Activated Protein Kinases
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Cell Adhesion / genetics
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Cell Division / physiology
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DNA-Binding Proteins
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Haploidy*
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Isoenzymes / genetics
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Isoenzymes / metabolism
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Membrane Glycoproteins
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Mutation
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Protein Subunits
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Trans-Activators / genetics
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Trans-Activators / metabolism
Substances
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DNA-Binding Proteins
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FLO11 protein, S cerevisiae
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Fungal Proteins
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Isoenzymes
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Membrane Glycoproteins
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Membrane Proteins
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NRG1 protein, S cerevisiae
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Nrg2 protein, S cerevisiae
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Protein Subunits
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Repressor Proteins
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SIP2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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SNF1-related protein kinases
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Protein Serine-Threonine Kinases
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SIP1 protein, S cerevisiae
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AMP-Activated Protein Kinases