Abstract
Elucidating the mechanism of ATP-dependent chromatin remodeling is one of the largest challenges in the field of gene regulation. One of the missing pieces in understanding this process is detailed structural information on the enzymes that catalyze the remodeling reactions. Here we use a combination of subunit radio-iodination and scanning transmission electron microscopy to determine the subunit stoichiometry and native molecular weight of the yeast SWI/SNF complex. We also report a three-dimensional reconstruction of yeast SWI/SNF derived from electron micrographs.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases
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Chromatin / metabolism*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / ultrastructure
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Image Processing, Computer-Assisted
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Iodine Radioisotopes
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Macromolecular Substances
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Microscopy, Electron, Scanning Transmission
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Models, Molecular
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Molecular Weight
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Nuclear Proteins*
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Protein Conformation
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / chemistry*
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Saccharomyces cerevisiae Proteins / metabolism*
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Saccharomyces cerevisiae Proteins / ultrastructure
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Transcription Factors / chemistry
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Transcription Factors / metabolism
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Transcription Factors / ultrastructure
Substances
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Chromatin
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DNA-Binding Proteins
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Iodine Radioisotopes
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Macromolecular Substances
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Nuclear Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Adenosine Triphosphatases
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SNF2 protein, S cerevisiae