Abstract
A ribosomal DNA (rDNA) binding activity was previously characterized in fission yeast that recognized the upstream ribosomal RNA (rRNA) gene promoter in a sequence specific manner and which stimulated rRNA synthesis. It was found to share characteristics with Saccharomyces cerevisiae's Upstream Activating Factor (UAF), an RNA polymerase I (pol I) specific transcription stimulatory factor. Putative fission yeast homologs of the S.cerevisiae UAF subunits, Rrn5p and Rrn10p, were identified. The Schizosaccharomyces pombe rDNA binding activity/transcriptional stimulatory activity was found to co-fractionate with both SpRrn5h and SpRrn10h. Analysis of polypeptides interacting with SpRrn10h uncovered a 27 kDa polypeptide (Spp27) homologous to a SWI/SNF component (now known to be homologous to Uaf30p). The contributions of the S.pombe and S.cerevisiae upstream rDNA promoter domains were assessed in cross-species transcriptional assays. Furthermore, comparative genomic analysis revealed putative Rrn5p, Rrn10p, Rrn9p and p27 homologs in multiple non-vertebrates. The S.pombe rDNA binding activity is proposed to be an RNA pol I specific SWI/SNF type factor.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism*
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Cell Cycle Proteins
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Cell Fractionation
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Chromosomal Proteins, Non-Histone
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DNA, Ribosomal / genetics
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DNA, Ribosomal / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Electrophoretic Mobility Shift Assay
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Molecular Sequence Data
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Protein Binding
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / genetics*
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Schizosaccharomyces pombe Proteins
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription, Genetic
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Two-Hybrid System Techniques
Substances
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Carrier Proteins
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Cell Cycle Proteins
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Chromosomal Proteins, Non-Histone
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DNA, Ribosomal
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DNA-Binding Proteins
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RRN10 protein, S cerevisiae
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RRN5 protein, S cerevisiae
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SWI1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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Transcription Factors
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swi1 protein, S pombe
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upstream activation factor, S cerevisiae
Associated data
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GENBANK/AL402119
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GENBANK/AL417122
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GENBANK/AL422400
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GENBANK/AL424621
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GENBANK/AL426913
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GENBANK/AL428395
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GENBANK/O14013
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GENBANK/Q10360