Abstract
In Saccharomyces cerevisiae the nicotinic acid moiety of NAD+ can be synthesized from tryptophan using the kynurenine pathway or incorporated directly using nicotinate phosphoribosyl transferase (NPT1). We have identified the genes that encode the enzymes of the kynurenine pathway and for BNA5 (YLR231c) and BNA6 (YFR047c) confirmed that they encode kynureninase and quinolinate phosphoribosyl transferase respectively. We show that deletion of genes encoding kynurenine pathway enzymes are co-lethal with the Deltanpt1, demonstrating that no other pathway for the synthesis of nicotinic acid exists in S. cerevisiae. Also, we show that under anaerobic conditions S. cerevisiae is a nicotinic acid auxotroph.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aerobiosis / physiology
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Anaerobiosis / physiology
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Gene Deletion
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Genes, Fungal
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Hydrolases / genetics
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Hydrolases / metabolism
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NAD / metabolism*
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Niacin / biosynthesis*
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Niacin / metabolism
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Pentosyltransferases / genetics
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Pentosyltransferases / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Sodium-Phosphate Cotransporter Proteins
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Sodium-Phosphate Cotransporter Proteins, Type III
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Symporters / physiology
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Tryptophan / metabolism
Substances
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Sodium-Phosphate Cotransporter Proteins
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Sodium-Phosphate Cotransporter Proteins, Type III
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Symporters
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NAD
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Niacin
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Tryptophan
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Pentosyltransferases
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nicotinate-nucleotide diphosphorylase (carboxylating)
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Hydrolases
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kynureninase
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nicotinate phosphoribosyltransferase